Effects of Rhizome Extracts (Motha) and Phytochemical Analysis on Antioxidant: A clinical study

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by Forest Raga February 22, 2021

 The present study was carried out to investigate the efficacy and safety of the Effects of Rhizome Extracts and Phytochemical Analysis on Antioxidant

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Chemicals and reagents

Aluminum chloride, ascorbic acid, chloroform, ethanol, ethyl acetate, ferric chloride, ferrous sulfate, gallic acid, glacial acetic acid, hexane, hydrogen peroxide, α-naphthol, petroleum ether, potassium dihydrogen phosphate, potassium hydrogen phosphate, potassium chloride, potassium ferricyanide, sodium bicarbonate, trichloroacetic acid (TCA), and vanillin were procured from SD Fine Chemicals Limited, Mumbai, India. Acetone, hydrochloric acid, methanol, sodium hydroxide, and sulfuric acid were acquired from Spectrum Chemical Private Limited, Cochin. Acetic anhydride, Dragendorff reagent, Folin€“Ciocalteu reagent, lead acetate, Mayer's reagent Sodium nitrate, and Wagner's reagent were purchased from MERCK Specialties Private Limited, Mumbai. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was procured from SISCO Research Laboratories, Maharashtra, India. Tris base was from NR Chemicals Private Limited, Mumbai, India. Thiobarbituric acid (TBA) was bought from Loba Chemie Private Limited, Mumbai, India. All chemicals and reagents were of analytical grade.

Collection and identification of plant material

The CRRs were obtained from the regional market, Bengaluru, and authenticated by National Ayurveda Dietetics Research Institute, Bengaluru. A specimen copy was deposited in the herbarium of the Regional Research Centre (RRCBI-AP. 77). The cleaned and shade-dried rhizomes were finely powdered using a mechanical blender (Kenstar, India) and were subjected to successive soxhlation using various solvents.


The yield of C. rotundus L. rhizome extracts

The yield of CRR extracts obtained after extraction with each solvent is given in Table 1. The highest yield of the extract was found to be in ETRE (22.72%) followed by MERE (3.22%). The lowest yield was obtained in PERE (0.133%).

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Preliminary phytochemical screening of CRR extracts

HERE, PERE and EARE extracts revealed the presence of alkaloids, carbohydrates, glycosides, and steroids. Flavonoids were not traced in CHRE extract. Saponins were present in ETRE, MERE, and AQRE whereas resins were present only in PERE and EARE extracts. CHRE showed the presence of tannins only. ACRE, ETRE, and MERE showed the presence of carbohydrates, glycosides, flavonoids, saponins, steroids, tannins, and phenols. Except HERE, PERE, and CHRE phenols were found in all the extracts [Table 2].

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This work is an effort to understand the medicinal potential of C. rotundus L. cultivated in the Karnataka region, India, for the first time. Successive soxhlation of C. rotundus L was carried out to identify the suitable solvent system for the extraction of numerous secondary metabolites and acetone was found to be a better solvent. The crude extract of the rhizome of CRR was observed to harbor substantial antioxidant activity, and the ACRE extract showed remarkable activity. The novel compound 1 (2)-Acetyl-3 (5)-styryl-5(3)-methylthiopyrazole, an alkaloid is reported for the first time. This work could provide a better understanding of phytochemicals present in C. rotundus and its antioxidative properties.

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